Structural Changes as a Tool for Affinity Recognition: Conformational Switch Biosensing

Tyrosine Phosphorylation as a Conformational Switch

Lalit Deshmukh, Nahum Meller, Nathan Alder , Tatiana Byzova, and Olga Vinogradova From the Departments of Pharmaceutical Sciences, School of Pharmacy and Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269, the Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio 44195, and the Laboratory of Chemical Physics, NI...

Converting a Binding Protein into a Biosensing Conformational Switch Using Protein Fragment Exchange

Biosensors can be used in applications ranging from identifying disease biomarkers to detecting spatial and temporal distributions of specific molecules in living cells. A major challenge facing biosensor development is how to functionally couple a biological recognition domain to an output module so that the binding event can be transduced to a visible and quantifiable signal [e.g., Förster re...

Aptamer conformational switch as sensitive electrochemical biosensor for potassium ion recognition.

We report the first use of an electrochemical aptasensor for selective potassium recognition, based on a conformational change, affording an electric signal transduced electrochemically by square wave voltammetry or electrochemical impedance spectroscopy.

Facial Expression Recognition Based on Structural Changes in Facial Skin

Facial expressions are the most powerful and direct means of presenting human emotions and feelings and offer a window into a persons’ state of mind. In recent years, the study of facial expression and recognition has gained prominence; as industry and services are keen on expanding on the potential advantages of facial recognition technology. As machine vision and artificial intelligence advan...

Microcalorimetry as a tool for structural biology MICROCALORIMETRY MOLECULAR STRUCTURE LABEL-FREE ANALYSIS BINDING AFFINITY